Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3′ consensus sequence AAUAAA

Abstract
A single U .fwdarw. G transversion in the 3'' consesus sequence AAUAAA of the adenovirus early region 1A gene was constructed and the effect for this mutation on processing of the 3'' end of the nuclear early region 1A RNA was analyzed. The results demonstrate that the intact AAUAAA is not required for RNA polyadenylation but is required for the cleavage step preceding polyadenylation to occur efficiently.