Concentration of sodium hyaluronate in serum

Abstract

A radioassay for sodium hyaluronate using high-affinity binding protein from bovinecartilage has been modified for serum analysis. The accuracy of the method was checked by isotope dilution experiments and by recovery studies with exogenous hyaluronate. The between-assay standard deviation in the determination is 15–20%. The concentration of sodiumhyaluronate in healthy adults (blood donors) is in the range of 10 to 100 μg/1 with a mean value in the order of 30 to 40 μg/l. This is a lower concentration than previously reported. The same level was found in young people. Higher hyaluronate concentrationswere noted in persons above 50 years of age. Analysis of plasma showed a slightly higheraverage hyaluronate level (5%) than in serum from the same persons. There were no notable sex differences. Analysis of serum and plasma from adult animals (rat, rabbit, dog, pig, goat, sheep, cow and horse) gave hyaluronate concentrations of the same order or higher than in human serum.