Insulin-induced translocation of glucose transporters from post-Golgi compartments to the plasma membrane of 3T3-L1 adipocytes.
Open Access
- 1 January 1988
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 106 (1), 69-76
- https://doi.org/10.1083/jcb.106.1.69
Abstract
A semiquantitative method using immunocytochemistry on ultrathin cryosections and the protein A-gold technique was performed to study the effect of insulin on the cellular distribution of the glucose transporters in cultured 3T3-L1 adipocytes. In basal cells a substantial portion of the label was present in a tubulovesicular structure at the trans side of the Golgi apparatus, likely to represent the trans-Golgi reticulum, and in small vesicles present in the cytoplasm. Treatment with insulin induced a rapid translocation of transporters from the tubulovesicular structure to the plasma membrane. The transporter labeling of the plasma membrane increased three-fold and that of the tubulovesicular structure decreased by half. There was no effect of insulin on the degree of label in the small cytoplasmic vesicles. Removal of insulin from stimulated cells rapidly reversed the distribution of transporters to that seen in basal cells. This study thus provides the first morphological evidence for the occurrence of transporter translocation in insulin action and identifies for the first time the intracellular location of the responsive transporters.This publication has 32 references indexed in Scilit:
- The trans Golgi Network: Sorting at the Exit Site of the Golgi ComplexScience, 1986
- Identification and partial purification of the insulin-responsive glucose transporter from 3T3-L1 adipocytesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- Possible pathways for lysosomal enzyme delivery.The Journal of cell biology, 1985
- Insulin-stimulated translocation of glucose transporters in the isolated rat adipose cells: Characterization of subcellular fractionsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1983
- Intracellular site of asialoglycoprotein receptor-ligand uncoupling: Double-label immunoelectron microscopy during receptor-mediated endocytosisCell, 1983
- Immunological identification of an insulin-responsive glucose transporterBiochemical and Biophysical Research Communications, 1982
- Detection of the rat adipose cell glucose transporter with antibody against the human red cell glucose transporterBiochemical and Biophysical Research Communications, 1982
- Organelle relationships in cultured 3T3-L1 preadipocytes.The Journal of cell biology, 1980
- A study of positive staining of ultrathin frozen sectionsJournal of Ultrastructure Research, 1978
- Reaction of proteins with glutaraldehydeArchives of Biochemistry and Biophysics, 1968