When a small circular area on the shoulder of a bovine host was treated with 1 ml of a hexane extract (200 male equivalents/ml) of fed male Amblyomma maculatum Koch, marked females released onto 3 other (separate) areas on the back of the animal migrated to the treated area and attached there. Moreover, the numbers that attached to the treated area increased as the distance of the treated area from site of release decreased. Likewise, when male extract was mixed with a small quantity of isobenzan (an insecticide) and applied to cattle, some of the released females migrated to the treated area, attached and were killed. Most females were lured by the attractant, received a toxic dose of insecticide, and died without attaching. Also, when the extract was applied to 1 ear of cattle (the preferred site of attachment of A. maculatum in the field) and the other ear was left untreated, over 4 times as many introduced females were attracted to and attached on the treated car as to the untreated ear at 1 day postinfestation.