Characterization and properties of a 5S‐RNA‐protein complex released from heated 60S ribosomal subunits
- 1 September 1984
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 143 (2), 303-307
- https://doi.org/10.1111/j.1432-1033.1984.tb08372.x
Abstract
When rat liver 60S ribosomal subunits were heated in phosphate buffer in the presence of MgCl2, 5S RNA was released in the form of a nucleoprotein complex (RNPH), which was isolated by electrophoresis in polyacrylamide gel or centrifugation through a sucrose gradient. In addition to L5 several proteins of functional significance were identified in the complex: the acidic phosphoproteins P1-P2 and, as weaker spots, L3-L4, L6-L7 and L22. Most of these proteins were also found, but only as traces, in the RNPEDTA used as a control. RNPH was able to associate with 40S subunits. Apparently, RNPH is located at the subunits'' interface, at or near the peptidyl-transferase center.This publication has 38 references indexed in Scilit:
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