Characterization of the keratan sulphate proteoglycans from bovine corneal stroma
- 1 March 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 169 (3), 517-530
- https://doi.org/10.1042/bj1690517
Abstract
The keratan sulphate proteoglycans that can be prepared from bovine corneal stroma [Axelsson & Heinegård (1975) Biochem. J. 145, 491-500] were characterized by gel chromatography, gel electrophoresis and analytical ultracentrifugation in associative (0.6 M-NaCl) and dissociative (6M-guanidinum chloride) solvents. The proteoglycans aggreagated at low salt concentrations and pH. The weight-average molecular weight of the monomer proteoglycans was established. Keratan sulphate peptides and oligosaccharide peptides were isolated after proteolysis. Their composition indicated that both are linked to protein via asparagine residues. A tentative model for corneal keratan sulphate proteoglycans is suggested.This publication has 31 references indexed in Scilit:
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