The Use of Molecular Probes to Distinguish New Primary Tumors from Recurrent Tumors in Gynecologic Malignancies
Open Access
- 1 October 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 94 (4), 432-434
- https://doi.org/10.1093/ajcp/94.4.432
Abstract
This is the first report using DNA molecular probe technology to distinguish between recurrent tumor and a second primary malignancy in a patient. Tumor DNA was extracted from squamous cell carcinoma of the cervix at the time of radical hysterectomy. Eighteen months later a squamous cell cancer was found in a vaginal apex biopsy from which DNA was extracted. Tumor DNA from both lesions was subjected to restriction enzyme digestion and DNA molecular hybridization with human papillomavirus (HPV) probes. Although both lesions were positive for HPV 16, their respective restriction enzyme patterns had different HPV genetic arrangements, thereby demonstrating their distinctness.Keywords
This publication has 5 references indexed in Scilit:
- HUMAN PAPILLOMAVIRUS GENOTYPE AS A PROGNOSTIC INDICATOR IN CARCINOMA OF THE UTERINE CERVIX1989
- APPLICATION OF DNA PROBES TO HEMATOLOGY - AN OVERVIEW WITH SELECTED EXAMPLES1989
- Identification of HPV 16 early genes retained in cervical carcinomaVirology, 1988
- Human papillomaviruses and cervical cancer: Analysis of histopathologic features associated with different viral typesHuman Pathology, 1988
- Human papillomavirus deoxyribonucleic acid in cervical carcinoma from primary and metastatic sitesAmerican Journal of Obstetrics and Gynecology, 1986