Evidence for an Essential Lysine at the Active Site of l‐Histidinol : NAD+ Oxidoreductase; a Bifunctional Dehydrogenase
- 1 August 1981
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 118 (1), 125-130
- https://doi.org/10.1111/j.1432-1033.1981.tb05494.x
Abstract
Histidinol dehydrogenase (EC 1.1.1.23) from Salmonella typhimurium is inhibited by formaldehyde and pyridoxal 5-phosphate (pyridoxal-P). epsilon-Pyridoxyl-lysine is isolated upon acid hydrolysis of pyridoxal-P-treated enzyme reduced by sodium borohydride. In the presence of formylhistidinol and formylhistidine (specific ligands of the enzyme) inactivation of histidinol dehydrogenase by pyridoxal-P is prevented. Extrapolation of the initial part of the inactivation curve caused by pyridoxal-P indicates that modification of two essential lysine residues results in inactivation of the dimeric enzyme. The essential lysine residues appear to participate in the reversible oxidation/reduction reaction converting histidinol to histidinal.Keywords
This publication has 28 references indexed in Scilit:
- Patterns of Product Inhibition of a Bifunctional Dehydrogenase; l‐Histidinol : NAD+ OxidoreductaseEuropean Journal of Biochemistry, 1981
- Histidinol dehydrogenase from Salmonella typhimurium and Escherichia coliBiochimica et Biophysica Acta (BBA) - Protein Structure, 1977
- Multifunctional ProteinsAnnual Review of Biochemistry, 1976
- Pyridoxal 5'-phosphate as a site-specific protein reagent for a catalytically critical lysine residue in rabbit muscle phosphoglucose isomeraseBiochemistry, 1971
- Uridine diphosphate glucose dehydrogenase of rat tissueBiochimica et Biophysica Acta (BBA) - Enzymology, 1971
- Synthesis and properties of α and ε pyridoxyl lysines and their phosphorylated derivativesBiochimie, 1971
- Purification and properties of UDPG dehydrogenase from beef liverArchives of Biochemistry and Biophysics, 1969
- Histidine regulatory mutants in Salmonella typhimuriumJournal of Molecular Biology, 1966
- Preparation of Crystalline Phosphorylated Derivatives of Vitamin B6Journal of the American Chemical Society, 1954
- Synthese von Polypeptiden XXVII. I. Derivate des HistidinsEuropean Journal of Organic Chemistry, 1908