The UNC-112 Gene in Caenorhabditis elegansEncodes a Novel Component of Cell–Matrix Adhesion Structures Required for Integrin Localization in the Muscle Cell Membrane
- 10 July 2000
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 150 (1), 253-264
- https://doi.org/10.1083/jcb.150.1.253
Abstract
Embryos homozygous for mutations in the unc-52, pat-2, pat-3, and unc-112 genes of C. elegans exhibit a similar Pat phenotype. Myosin and actin are not organized into sarcomeres in the body wall muscle cells of these mutants, and dense body and M-line components fail to assemble. The unc-52 (perlecan), pat-2 (alpha-integrin), and pat-3 (beta-integrin) genes encode ECM or transmembrane proteins found at the cell-matrix adhesion sites of both dense bodies and M-lines. This study describes the identification of the unc-112 gene product, a novel, membrane-associated, intracellular protein that colocalizes with integrin at cell-matrix adhesion complexes. The 720-amino acid UNC-112 protein is homologous to Mig-2, a human protein of unknown function. These two proteins share a region of homology with talin and members of the FERM superfamily of proteins. We have determined that a functional UNC-112::GFP fusion protein colocalizes with PAT-3/beta-integrin in both adult and embryonic body wall muscle. We also have determined that UNC-112 is required to organize PAT-3/beta-integrin after it is integrated into the basal cell membrane, but is not required to organize UNC-52/perlecan in the basement membrane, nor for DEB-1/vinculin to localize with PAT-3/beta-integrin. Furthermore, UNC-112 requires the presence of UNC-52/perlecan and PAT-3/beta-integrin, but not DEB-1/vinculin to become localized to the muscle cell membrane.Keywords
This publication has 47 references indexed in Scilit:
- The Genome Sequence of Drosophila melanogasterScience, 2000
- The FERM domain: a unique module involved in the linkage of cytoplasmic proteins to the membraneTrends in Biochemical Sciences, 1998
- The Caenorhabditis elegans gene unc-89, required fpr muscle M-line assembly, encodes a giant modular protein composed of Ig and signal transduction domains.The Journal of cell biology, 1996
- Regulation of cell adhesion and anchorage-dependent growth by a new β1-integrin-linked protein kinaseNature, 1996
- Chapter 19 DNA TransformationPublished by Elsevier ,1995
- Green Fluorescent Protein as a Marker for Gene ExpressionScience, 1994
- Integrins: Versatility, modulation, and signaling in cell adhesionCell, 1992
- Vinculin is essential for muscle function in the nematode.The Journal of cell biology, 1991
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Muscle organization in Caenorhabditis elegans: localization of proteins implicated in thin filament attachment and I-band organization.The Journal of cell biology, 1985