RNA substrate binding site in the catalytic core of the Tetrahymena ribozyme
- 1 July 1992
- journal article
- Published by Springer Nature in Nature
- Vol. 358 (6382), 123-128
- https://doi.org/10.1038/358123a0
Abstract
In catalysis by group I introns, the helix (P1) containing the RNA cleavage site must be positioned next to the guanosine binding site. We have identified a conserved adenine in the catalytic core that contributes to the stability of this arrangement and propose that it accepts a hydrogen bond from a specific 2'-OH in P1. Such base-backbone tertiary interactions may be generally important to the organization of RNA tertiary structure.Keywords
This publication has 44 references indexed in Scilit:
- DNA cleavage catalysed by the ribozyme from TetrahymenaNature, 1990
- The guanosine binding site of the Tetrahymena ribozymeNature, 1989
- Sequence-specific endoribonuclease activity of the Tetrahymena ribozyme: enhanced cleavage of certain oligonucleotide substrates that form mismatched ribozyme-substrate complexesBiochemistry, 1988
- Kinetics for reaction of a circularized intervening sequence with CU, UCU, CUCU, and CUCUCU: mechanistic implications from the dependence on temperature and on oligomer and magnesium concentrationsBiochemistry, 1988
- The Tetrahymena ribozyme acts like an RNA restriction endonucleaseNature, 1986
- One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activityCell, 1986
- Splice-site selection by a self-splicing RNA of TetrahymenaNature, 1986
- Reversibility of cyclization of the tetrahymena rRNA intervening sequence: implication for the mechanism of splice site choiceCell, 1985
- Making ends meet: a model for RNA splicing in fungal mitochondriaNature, 1982
- Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structureBiochimie, 1982