Magnetization-transfer NMR Investigation of the Hydrogen Exchange in Mixtures of N-Methylacetamide and Water.
- 1 January 1983
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 37b (3), 227-234
- https://doi.org/10.3891/acta.chem.scand.37b-0227
Abstract
Hydrogen exchange rates in mixtures of N-methylacetamide [NMA] and water are measured by the magnetization-transfer method. The exchange rate is studied as a function of pH, concentration and temperature. In mixtures with the mol ratio of NMA-H2O = 1:5.16, at 351 K, the exchange is catalyzed by protons and by hydroxyl ions; at pH = 5.0, where the rate is minimal, the uncatalyzed exchange between the peptide group and H2O amounts to 75% of the total exchange measured. The results obtained are discussed with particular reference to the hydrogen exchange in protein solutions.This publication has 4 references indexed in Scilit:
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- Kinetics of the exchange of individual amide protons in the basic pancreatic trypsin inhibitorJournal of Molecular Biology, 1979
- Packing defects, cavities, volume fluctuations, and access to the interior of proteins. Including some general comments on surface area and protein structureCarlsberg Research Communications, 1979
- Nuclear magnetic resonance measurement of hydrogen exchange kinetics of single protons in basic pancreatic trypsin inhibitorBiochemistry, 1978