Replication of the Human Hepatitis Delta Virus Genome Is Initiated in Mouse Hepatocytes following Intravenous Injection of Naked DNA or RNA Sequences
- 1 April 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 75 (7), 3469-3473
- https://doi.org/10.1128/jvi.75.7.3469-3473.2001
Abstract
As early as 5 days after DNA copies of the hepatitis delta virus (HDV) genome or even in vitro-transcribed HDV RNA sequences were injected into the mouse tail vein using the hydrodynamics-based transfection procedure of F. Liu et al. (Gene Ther. 6:1258–1266, 1999), it was possible to detect in the liver by Northern analyses of RNA, immunoblots of protein, and immunostaining of liver sections what were considered typical features of HDV genome replication. This transfection strategy should have valuable applications for in vivo studies of HDV replication and pathogenesis and may also be useful for studies of other hepatotropic viruses.Keywords
This publication has 59 references indexed in Scilit:
- Efficient Site-Specific Nonribozyme Opening of Hepatitis Delta Virus Genomic RNA in Infected LiversJournal of Virology, 2000
- Limitations to Replication of Hepatitis Delta Virus in Avian CellsJournal of Virology, 2000
- Long-term expression of human alpha1-antitrypsin gene in mouse liver achieved by intravenous administration of plasmid DNA using a hydrodynamics-based procedureGene Therapy, 2000
- Interactions between Hepatitis Delta Virus ProteinsJournal of Virology, 2000
- Sustained survival of human hepatocytes in mice: A model for in vivo infection with human hepatitis B and hepatitis delta virusesNature Medicine, 2000
- Hydrodynamics-based transfection in animals by systemic administration of plasmid DNAGene Therapy, 1999
- High Levels of Foreign Gene Expression in Hepatocytes after Tail Vein Injections of Naked Plasmid DNAHuman Gene Therapy, 1999
- Hepatitis delta virus mutant: effect on RNA editingJournal of Virology, 1995
- Structure and replication of the genome of the hepatitis delta virus.Proceedings of the National Academy of Sciences, 1986
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970