Procedure for mutagenizing spores of Saccharomyces cerevisiae
- 1 November 1983
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 156 (2), 907-908
- https://doi.org/10.1128/jb.156.2.907-908.1983
Abstract
A procedure for inducing mutants of a homothallic strain of Saccharomyces cerevisiae is described. The essential parts of the procedure are long incubation in Glusulase, which preferentially kills vegetative cells instead of spores, and treatment in 9% ethyl methanesulfonate, which also preferentially kills vegetative cells instead of spores. Consequently, the viable population is virtually 100% spores.Keywords
This publication has 7 references indexed in Scilit:
- Random-spore analysis in Saccharomyces cerevisiaeHereditas, 2009
- Chapter 8 Preparation and Growth of Yeast ProtoplastsMethods in Cell Biology, 1975
- [3] The biochemical genetics of yeastPublished by Elsevier ,1970
- Preparation and Storage of Single Spores of Saccharomyces cerevisiaeJournal of Bacteriology, 1969
- An Enrichment Method for Auxotrophic Yeast Mutants using the Antibiotic ‘Nystatin’Nature, 1966
- GENETICAL MUTANTS INDUCED BY ETHYL METHANESULFONATE INSACCHAROMYCESCanadian Journal of Genetics and Cytology, 1965
- USE OF SNAIL DIGESTIVE JUICE IN ISOLATION OF YEAST SPORE TETRADSJournal of Bacteriology, 1959