Localization of mammary tumors in vivo with131i-labeled fab fragments of antibodies against mouse mammary epithelial (mme) antigens

Abstract

The Fab fragments of antibodies against cell-type-specific surface antigens of mouse mammary epithelial cells (MME-antigens) were used to localize mammary tumors successfully. The radioiodine-labeled anti-MME (Fab) was injected into mice carrying simulated mammary metastases, and after 24 hours the amount of label per gram of excised tissue was several times greater in the tumor than in liver, brain, lung, or muscle. Kidney showed considerable accumulation of label but this appeared to be nonspecific. Kinetic studies revealed a rapid elimination of labeled Fab in the urine with only 1% of the injected dose remaining in the entire blood pool after 24 hours. With a high-purity germanium camera, mammary tumors were clearly located by the ¹³¹I-labeled anti-MME (Fab), and normalization to ^99mTc-pertechnetate distribution in the animal increased the specificity. The density of ¹³¹I-label was fourfold greater over the mammary tumor than over comparable areas of the mouse. No accumulation of ¹³¹I-anti-MME (Fab) was observed in nonmammary tumors nor in mammary tumors when labeled nonspecific Fab was used. An analogous system using an antihuman mammary epithelial antiserum is being developed for localization of breast metastases in humans.