Molecular cloning of a levocabastine‐sensitive neurotensin binding site
- 20 May 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 386 (2-3), 91-94
- https://doi.org/10.1016/0014-5793(96)00397-3
Abstract
A search for sequences homologous to the neurotensin receptor cDNA in a rat hypothalamic library has identified a novel neurotensin receptor (NTR-2). The 1539 bp cDNA encodes a 416 amino acid protein and shows highest homology to the previously cloned neurotensin receptor (NTR-1) (64% homology and 43% identity). Binding and pharmacological studies demonstrate that NTR-2 expressed in COS cells recognizes neurotensin (NT) with high affinity as well as several other agonists and antagonists. However, a fundamental difference was found; unlike NTR-1, NTR-2 recognizes, with high affinity, levocabastine, a histamine H1 receptor antagonist previously shown to compete with NT for low-affinity binding sites in brain.Keywords
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