Role of Magnesium in the Enzyme-Linked Immunosorbent Assay for Lipopolysaccharides of Rough Escherichia coli Strain J5 and Neisseria gonorrhoeae

Abstract

An enzyme-linked immunosorbent assay for antibody to the lipopolysaccharides (LPSs) of a rough strain of Escherichia coli (J5) and Neisseria gonorrhoeae was developed. When standard methods of this assay were applied to these LPSs, no antibody was measured. By radiolabeling smooth and rough endotoxins with ⁵¹Cr, it was discovered that the rough LPS was not adhering to the polystyrene tubes. When Mg++ was added, however, all endotoxins adsorbed to the solid phase. With this assay antiserum to core glycolipid had higher titers against its smooth parent strain than did homologous antiserum, and antisera to gonococci had high homologous but low heterologous titers. Thus, these data demonstrate that bulky O side chains do not hinder the penetration of antibody to core glycolipid and that LPSs from gonococci are heterogeneous but do cross-react.