MODIFICATION OF PULMONARY VASCULAR-RESPONSES TO ARACHIDONIC-ACID BY ALTERATIONS IN PHYSIOLOGIC STATE

Abstract

The effects of bolus injections of arachidonic acid and prostaglandins (PG) E2 and F2.alpha. on the pulmonary vascular bed were compared under resting conditions and after alteration in the physiologic state of the lung. The vascularly isolated lung lobe of the intact, anesthetized dog was studied under conditions of controlled blood flow. Arachidonic acid, PGE2 and PGF2.alpha. increased pulmonary vascular resistance by constricting intrapulmonary veins and arteries in a dose-related manner, as did an analog of the endoperoxide, PGH2, whereas PGI2 dilated the pulmonary vascular bed. The response to arachidonate was associated with a 2- to 3-fold increase in levels of PGE- and PGF-like substances in pulmonary venous blood and was blocked by indomethacin. The effects of arachidonic acid, but not the PG, were greatly enhanced during perfusion with either dextran or saline and the enhanced response in saline was associated with a 15-20-fold increase in levels of PG-like substances in the pulmonary effluent. Responses to arachidonate were not dependent upon the presence of formed elements in blood but were related to perfusate protein concentration. Alveolar hypoxia decreased responses to the precursor while those to PGE2 and PGF2.alpha. were enhanced. Responses to the PG, but not those to arachidonate, were affected by changes in blood pH. Sublethal doses of Escherichia coli endotoxin increased the response to arachidonic acid, but not those to PGE2 and PGF2.alpha.. The effects of bolus injection of arachidonic acid on the pulmonary vascular bed apparently are due mainly to formation of constrictor metabolites which may overshadow the actions of any dilator (PGI2) formed; metabolism of the precursor apparently is altered by changes in physiologic state.