A modified mouse peripheral blood lymphocyte culture system for cytogenetic analysis
- 1 January 1987
- journal article
- research article
- Published by Wiley in Environmental and Molecular Mutagenesis
- Vol. 10 (4), 377-386
- https://doi.org/10.1002/em.2850100407
Abstract
A detailed methodology is presented for culturing mouse peripheral blood lymphocytes isolated on density gradients and stimulated to divide using either phytohem-agglutinin, concanavalin A, or lipopolysaccharide. The techniques described yield more than sufficient numbers of mitotic cells for analyzing sister chromatid exchange, chromosome aberrations, and micronuclei following in vitro or in vivo exposure to chemicals or radiation.Keywords
This publication has 20 references indexed in Scilit:
- Diaziquione-induced micronuclei in cytochalasin B-blocked mouse peripheral blood lymphocytesMutation Research, 1987
- Sister chromatid exchange analysis in lung and peripheral blood lymphocytes of mice exposed to methyl isocyanate by inhalationEnvironmental Mutagenesis, 1987
- Genotoxicity studies of benz[l]aceanthryleneCancer Letters, 1986
- Induction of sister-chromatid exchange (SCE) and cell-cycle inhibition in mouse peripheral blood B lymphocytes exposed to mutagenic carcinogens in vivoMutation Research/Genetic Toxicology, 1985
- Simultaneous assessment of ethyl carbamate-induced SCEs in murine lymphocytes, bone marrow and alveolar macrophage cellsCarcinogenesis: Integrative Cancer Research, 1984
- Development of Rodent Peripheral Blood Lymphocyte Culture Systems to Detect Cytogenetic Damage In VivoPublished by Springer Nature ,1984
- Analyses of sister-chromatid exchange and cell-cycle kinetics in mouse T- and B-lymphocytes from peripheral blood culturesMutation Research, 1983
- A method for preparing chromosomes from peripheral blood of the mouseMutation Research Letters, 1982
- Aging and sister chromatid exchange. VIII. Effect of the aging environment on sister chromatid exchange induction and cell cycle kinetics in Ehrlich ascites tumor cells. A brief noteMechanisms of Ageing and Development, 1981
- Suppression of Immunity by Stress: Effect of a Graded Series of Stressors on Lymphocyte Stimulation in the RatScience, 1981