The half-life of mRNA in Saccharomyces cerevisiae
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 170 (2), 137-144
- https://doi.org/10.1007/bf00337788
Abstract
The decay kinetics of mRNA was studied in a yeast temperature-sensitive mutant, ts136, which is defective in cytoplasmic RNA production at 37° C. The disappearance of the synthetic capacity of mRNA was determined by withdrawing equal volumes of ts136 cell culture and pulse-labelling with [35S]methionine at various time intervals after the shift to 37° C from 23° C. The synthesized proteins were separated on a two-dimensional gel electrophoretic system and then quantitatively analyzed for their incorporated radioactivities by scintillation counting. Our results show that yeast mRNAs have divergent functional half-lives ranging from 4.5 to 41 min, with an average value of 22 min. Each mRNA exhibits a simple exponential decay with its own characteristic decay pattern. Of the approximately 500 major polypeptides made by yeast cells, which are detectable on autoradiograms of the gels, 80 were arbitrarily selected and the mRNAs coding for those polypeptides were examined for their decay kinetics.This publication has 29 references indexed in Scilit:
- The metabolism of poly(A)+ and poly(A)− hnRNA in cultured drosophila cells studied with a rapid uridine pulse-chaseCell, 1977
- The synthesis of eucaryotic ribosomal proteins in vitroCell, 1977
- Methylated, blocked 5' termini of yeast mRNA.Journal of Biological Chemistry, 1976
- Coordinate regulation of the synthesis of eukaryotic ribosomal proteins.Proceedings of the National Academy of Sciences, 1976
- Half life of yeast messenger RNANature, 1976
- High resolution two-dimensional electrophoresis of proteins.1975
- Messenger RNA turnover in mouse L cellsJournal of Molecular Biology, 1973
- Lomofungin, an Inhibitor of Ribonucleic Acid Synthesis in Yeast Protoplasts: Its Effect on Enzyme FormationAntimicrobial Agents and Chemotherapy, 1973
- The effect of cycloheximide upon polyribosome stability in two yeast mutants defective respectively in the initiation of polypeptide chains and in messenger RNA synthesisMolecular Genetics and Genomics, 1970