Simian virus 40-specific proteins in the membranes of simian virus 40-transformed hamster and mouse cells.

Abstract

Membranes of SV-40-transformed hamster lymphocytes and phagocytes, and of transformed mouse fibroblasts, contain 2 classes of antigenic virus-specific protein. The isoelectric points of these proteins, as defined by isoelectric focusing/immune electrophoresis are at pH 4.5 and 4.7. MW of the pI [isoelectric point] 4.5 and pI 4.7 components, determined by isoelectric focusing/dodecyl sulfate polyacrylamide electrophoresis, lie near 58,000 and 90,000-110,000, respectively. The pI 4.5 and pI 4.7 proteins are tentatively identified with the surface (transplantation) and U antigens, respectively.