Structure and Development of Vesicles in Larval Tissue Culture of Aedes Aegypti (L.)

Abstract

Structure and development of vesicles in larval tissue culture of AEDES AEGYPTI have been studied. The vesicles are made up of a single layer of hexa- or pentagonal epithelial type of cells. The outer surface of these vesicles is covered with a thin cuticlelike membrane. In freshly set up cultures the vesicles develop as small bubble-like outgrowths from the larval tissue. Further enlargement of these vesicles takes place by an increase in the number of cells by cell division. New vesicles arise from the older vesicles or their fragments attached to the glass as small cytoplasmic vacuoles in 1 of the cells and by cell division from multicellular vesicles. The A. aegypti vesicles have been subcultured. Two separate lines of vesicles are being maintained.