13C Nuclear Magnetic Resonance Spectra of Proteins

Abstract

Although ¹³C has a natural abundance of only 1.1% and gives inherently much weaker nuclear magnetic resonance signals than does does ¹H, it has been found that ¹³C nuclear magnetic resonance spectra of aqueous solutions of naturally occurring proteins can be observed. A spectrum of hen's egg white lysozyme, an enzyme of molecular weight approximately 14 300, containing 129 amino acid residues, has been partially analyzed by comparison with a computer—simulated spectrum.