Effect of polyamines on the stability of brain-cortex ribosomes
- 1 October 1969
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 114 (4), 847-854
- https://doi.org/10.1042/bj1140847
Abstract
1. Ribosomes isolated from the cortex tissue of goat brain contain very small amounts of spermidine and spermine. Ribosomes isolated from spermidine-treated slices have a higher spermidine content. 2. The polyamines partially prevent the temperature-dependent breakdown of ribosomes into acid-soluble nucleotides. 3. The ‘melting’ temperature of ribosomes rises slightly when the ribosomes are heated slowly in the presence of polyamines. 4. The pH-dependent breakdown of ribosomes into protein, RNA and acid-soluble nucleotide is markedly decreased by polyamines present in media in which ribosomes are suspended. 5. The breakdown of ribosomes in the presence of high concentrations of salts and EDTA is partially checked by the concurrent presence of polyamines. 6. Spermidine and spermine make ribosomes less susceptible to enzymic digestion by crystalline trypsin and ribonuclease.Keywords
This publication has 30 references indexed in Scilit:
- Ribonuclease and trypsin treatment of ribosomes and polyribosomes from sea urchin eggsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- Effect of polyamines on ribosomal RNA synthesis during sea urchin developmentExperimental Cell Research, 1968
- Influence of growing Aerobacter Aerogenes in a polyamine-enriched medium on the stability of the 70S ribosomesExperimental Cell Research, 1967
- Brain RibosomesBrain Research, 1966
- A study of ribosomes and of ribonucleic acid from a thermophilic organismBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
- Spermidine and Spermine in Rat Tissues at Different AgesActa Physiologica Scandinavica, 1964
- RIBONUCLEASE ACTIVITY OF GOAT BRAIN CORTEX RIBOSOMAL PREPARATIONJournal of Neurochemistry, 1964
- A CYTOPHYSIOLOGICAL STUDY OF THE FUNCTIONAL RELATIONSHIP BETWEEN OLIGODENDROGLIAL CELLS AND NERVE CELLS OF DEITERS' NUCLEUSJournal of Neurochemistry, 1960
- The quantitative histochemistry of brain. II. Enzyme measurements.1954
- The colorimetric determination of phosphorusBiochemical Journal, 1932