Renal sulfate transport at the basolateral membrane is mediated by anion exchange.
- 1 May 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (9), 2603-2607
- https://doi.org/10.1073/pnas.80.9.2603
Abstract
The transport of sulfate was studied in basolateral membrane (BLM) vesicles isolated from rat kidney cortex by centrifugation on a Percoll self-generating gradient. In contrast to sulfate transport at the luminal membrane, sulfate uptake by BLM vesicles was not Na dependent. Imposition of an inside > outside HCO3- gradient stimulated BLM sulfate uptake nearly 10-fold and produced a transient overshoot of about 4-fold. This process appeared to be come saturated at high concentrations of either HCO3- or sulfate. Sulfate itself, thiosulfate, and H- but not Cl or thiocyanate, were able to substitute for HCO3-. None of these anions was as effective as HCO3-. The sulfate/HCO3 exchange was unaltered by manipulation of membrane potential, suggesting that it was electroneutral. Both HCO3- stimulation and overshoot could be prevented by known inhibitors of anion transport, including mercuric chloride, 4-acetamido-4''-isothiocyanostilbene-2,2''-disulfonic acid, and phloretin. HCO3--stimulated sulfate uptake also was inhibited by thiosulfate, probenecid, and acetazolamide. Rat kidney BLM vesicles showed carrier-mediated anion exchange; this carrier may participate in both reabsorptive and secretory sulfate transport.This publication has 31 references indexed in Scilit:
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