Collections of fishes from the several bodies of fresh and brackish waters on Sable Island, Nova Scotia (60°06′W, 43°56′N), were composed variously of four euryhaline species: Fundulus heteroclitus (Cyprinodontidae), Gasterosteus wheatlandi, Apeltes quadracus, and Pungitius pungitius (Gasterosteidae). Sight records are reported for Anguilla rostrata (Anguillidae).