Purification and Properties of the Membrane Form of Variant Surface Glycoproteins (VSGs) from Trypanosoma brucei1,2,3
- 1 February 1984
- journal article
- research article
- Published by Wiley in The Journal of Protozoology
- Vol. 31 (1), 53-60
- https://doi.org/10.1111/j.1550-7408.1984.tb04289.x
Abstract
Variant surface glycoproteins (VSG) of Trypanosoma brucei are released in a water soluble form on impairment of membrane integrity. We have previously shown that this release is the result of an enzyme-mediated event which converts the hydrophobic membrane form VSG into the hydrophilic water-soluble form. We now present further details of the methods by which membrane form VSG ( mfVSG ) may be isolated, uncontaminated by water-soluble VSG ( sVSG ). The sensitivity to different metal ions of the enzyme that mediated the conversion event is discussed, and some biochemical characteristics of different mfVSG preparations are presented.Keywords
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