Collection and cryopreservation of semen was undertaken in a 40-year-old man undergoing combinalion cyclophosphamide-doxorubicin-vinicristine-prednisone chemotherapy for diffuse large cell lymphoma. Semen samples worthy of cryopreservation could be obtained through 39 days of therapy, with azoospermia observed after 70 days. Although pregnancy has not been attempted in the current case, reports by others suggest that normal outcomes can be expected with thawed semen collected during chemotherapy.