Bacteriophage T4 deoxynucleotide kinase: gene cloning and enzyme purification
Open Access
- 1 June 1990
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 172 (6), 2935-2939
- https://doi.org/10.1128/jb.172.6.2935-2939.1990
Abstract
Gene 1 of bacteriophage T4 has been cloned into a lambda pL expression vector, resulting in the overproduction of deoxynucleotide kinase. A procedure that includes affinity chromatography on Cibacron Blue F3GA-agarose has been used to purify milligram quantities of enzymes from a 2-liter culture. The enzyme has been partially characterized in vitro and in vivo, and it appears to be identical to the deoxynucleotide kinase isolated from T4-infected Escherichia coli. These results prove the earlier contention that the phosphorylation of three dissimilar deoxynucleotides (5-hydroxymethyldeoxycytidylate, dTMP, and dGMP), to the exclusion of most others, is catalyzed by a single protein.This publication has 27 references indexed in Scilit:
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