ELECTROPHORETIC AND CHEMICAL ANALYSIS OF PROTEIN IN NEPHRITIC URINE 1

Abstract

The relative % of globulin in a series of 22 twenty-four-hr. specimens of urine from 5 nephritic patients was detd. by a chemical method and by the electrophoretic moving boundary method of Tiselius. The conc. of total protein in the specimens varied from 0.450-3.45 gs. %. The results obtained by the 2 methods agreed closely. The conc. of [gamma] globulin was high in the urine of 2 patients with rapidly progressing nephrotic nephritis, lower in the urine of a patient with slowly progressing nephrotic nephritis, and was very low in the urine of a child with lipid nephrosis and normal renal func- tion. No fibrinogen was found in the urine of the cases of nephrotic nephritis. This confirms previous conclusions that the hyaline coagula which collect in glomeruli and tubules of such cases are probably derived from globulins other than fibrinogen. The Bence-Jones protein in the urine of a patient with multiple myeloma and progressive renal insufficiency behaved electrophoretically like a B globulin.