Continuous Wave Diode Laser Induced Two-Photon Fluorescence Excitation of Three Calcium Indicators
Open Access
- 1 December 1997
- journal article
- Published by IOP Publishing in Japanese Journal of Applied Physics
- Vol. 36 (12A), L1598
- https://doi.org/10.1143/jjap.36.l1598
Abstract
Continuous wave (CW) diode laser induced two-photon fluorescence spectra have been measured for calcium indicators of Indo-1, Oregon-green, and Fluo-3. The near 2.0 log-log power-dependence of fluorescence intensity versus incident laser power indicates that the fluorescence from these three calcium indicators was excited by two-photon absorption process of 809-nm laser light. Calcium-dependent emission spectra of Indo-1 and Oregon-green for two-photon excitation under wavelength of 809-nm have also been measured.Keywords
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