Morphological changes in the surface characteristics of cultured cells after exposure to diagnostic ultrasound.

Abstract

Morphological changes in the surface membranes of cultured mouse Balb/c 3T3, clone 1-13 cells were studied by scanning electron microscopy after the cells were exposed to diagnostic ultrasound. Abundant irregular, dense microvilli appeared in transformed clones and in cells that were exposed to ultrasound, but had not been morphologically transformed. This increased density of microvilli was detected as early as 3 days after ultrasound and was still present 37 days later. Unexposed control cells were smooth with occasional microvilli. Further studies are needed of the effects of diagnostic ultrasound.