Carbon Dioxide-Induced Oscillations in Fluorescence and Photosynthesis
- 1 December 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 88 (4), 1125-1130
- https://doi.org/10.1104/pp.88.4.1125
Abstract
The response of CO2 fixation to a sudden increase in ambient CO2 concentration has been investigated in intact leaf tissue from spinach (Spinacia oleracea) using a dual channel infrared gas analyzer. Simultaneous with these measurements, changes in fluorescence emission associated with a weak, modulated measuring beam were recorded. Application of brief (2-3 seconds) dark intervals enabled estimation of the dark fluorescence level (Fo) under both steady state and transient conditions. The degree of supppression of Fo level fluorescence in the light was strongly correlated with nonphotochemical quenching under all conditions. During CO2-induced oscillations in photosynthesis under 2% O2 the changes in nonphotochemical quenching anticipate changes in the rate of uptake of CO2. At such low levels of O2 and constant illumination, changes in the relative quantum efficency of open photosystem II units were estimated as the ratio of the rate of CO2 uptake and the photochemical quenching coefficient. Under the same conditions the relative quantum efficiency of photosystem II was found to vary inversely with the degree of nonphotochemical quenching. The relationship between changes in the rate of CO2 uptake:photochemical quenching coefficient and nonphotochemical quenching was altered somewhat when the same experiment was conducted under 20% O2. The results suggest that electron transport coupled to reduction of O2 occurs to varying degrees with time during oscillations, especially when ambient O2 concentrations are high.This publication has 15 references indexed in Scilit:
- Relationship between Steady-State Fluorescence Yield and Photosynthetic Efficiency in Spinach Leaf TissuePlant Physiology, 1988
- Photoinhibition and Zeaxanthin Formation in Intact LeavesPlant Physiology, 1987
- Oscillations in levels of metabolites from the photosynthetic carbon reduction cycle in spinach leaf disks generated by the transition from air to 5% CO2Archives of Biochemistry and Biophysics, 1986
- A Numerical Approach to Measurement of CO2 Exchange Transients by Infrared Gas AnalysisPlant Physiology, 1984
- Assimilatory Power (Postillumination CO2 Uptake) in LeavesPlant Physiology, 1984
- Some effects of CO 2 concentration and decreased O 2 concentration on induction fluorescence in leavesProceedings of the Royal Society of London. B. Biological Sciences, 1983
- Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Resolution into two componentsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1982
- Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of Photosystem II electron acceptors and fluorescence emission from Photosystems I and IIBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1981
- Regulation of photosynthetic electron transport and photophosphorylation in intact chloroplasts and leaves of Spinacia oleracea L.Planta, 1978
- Fluorescence quenching in Photosystem II of chloroplastsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1975