Calf-Ovary Protein Kinases Dependent on Adenosine 3': 5'-Monophosphate. Analysis by Electrophoresis and Electro-Focusing on Polyacrylamide Gel
- 1 March 1977
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 73 (2), 401-409
- https://doi.org/10.1111/j.1432-1033.1977.tb11331.x
Abstract
High resolving power and quantitative application of polyacrylamide-gel electrophoresis at various pore sizes and electrofocusing provide resolution of a calf-ovarian protein kinase [EC 2.7.1.37] system at an increased level of magnification, as well as optimal preparative routes. Three protein kinases dependent on cyclic AMP are distinguished by polyacrylamide gel electrophoresis in calf ovarian cytosol. These enzymes which are observed in the pH range 7.5-10.2, appear to be aggregates of a common subunit or monomer. The 3 kinases are, by the criteria of polyacrylamide gel electrophoresis, distinct from 3 cyclic AMP-binding proteins found in the calf ovarian system. Analysis by electrofocusing on polyacrylamide gel shows that conventionally purified preparations of the major kinase of cytosol contain an overwhelming majority of contaminant proteins.This publication has 36 references indexed in Scilit:
- Characterization of Calf‐Ovary Adenosine‐3′: 5′‐Monophosphate‐Dependent Protein Kinases and Adenosine‐3′: 5′‐Monophosphate‐Binding ProteinsEuropean Journal of Biochemistry, 1975
- A simple electronic device for the measurement of band migration distances in polyacrylamide gelsAnalytical Biochemistry, 1975
- TSH regulation of cAMP-dependent protein kinase activity in the thyroidBiochemical and Biophysical Research Communications, 1974
- Protein Kinase Translocation as an Early Event in the Hormonal Control of Uterine ContractionScience, 1974
- Mechanism of Action of Gonadotropin. III. Binding of Adenosine 3′,5′-Monophosphate by the 105,000 × g Supernatant Fraction from Homogenates of Calf Ovaries1Endocrinology, 1973
- Studies on the Redox State in Polyacrylamide GelsSeparation Science, 1972
- Staining of protein zones after isoelectric focusing in polyacrylamide gelsBiochimica et Biophysica Acta (BBA) - Protein Structure, 1971
- Anti-estradiol antibodies: Isoelectric focusing in polyacrylamide gelAnalytical Biochemistry, 1971
- Polyacrylamide Gel ElectrophoresisScience, 1971
- Prostaglandin Receptor Site: Evidence for an Essential Role in the Action of Luteinizing HormoneScience, 1970