Allelic exclusion in transgenic mice carrying mutant human IgM genes.
Open Access
- 1 June 1988
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 167 (6), 1969-1974
- https://doi.org/10.1084/jem.167.6.1969
Abstract
Expression of the membrane-bound version of the human mu chain in transgenic mice results in the allelic exclusion of endogenous mouse Ig heavy chain genes (6). The secreted version of the human Ig transgene has no such effect. F1 hybrid animals that carry transgenes for both secreted and membrane-bound human mu chains produce both forms of the human heavy chain while strongly suppressing endogenous mouse mu expression. The simultaneous expression of the two rearranged transgenes in primary B cells suggests that allelic exclusion operates before the formation of a second functionally rearranged heavy chain gene in vivo.Keywords
This publication has 21 references indexed in Scilit:
- Expression of immunoglobulin delta chain causes allelic exclusion in transgenic miceNature, 1987
- Allelic Exclusion in Transgenic Mice That Express the Membrane form of Immunoglobulin μScience, 1987
- Transgenic mice with mu and kappa genes encoding antiphosphorylcholine antibodies.The Journal of Experimental Medicine, 1986
- A transgenic immunoglobulin Mu gene prevents rearrangement of endogenous genesCell, 1985
- Transmission and expression of a specific pair of rearranged immunoglobulin μ and κ genes in a transgenic mouse lineNature, 1985
- Introduction of a μ immunoglobulin gene into the mouse germ line: Specific expression in lymphoid cells and synthesis of functional antibodyCell, 1984
- Analysis of B Lymphocyte Differentiation Antigens by Flow MicrofluorometryImmunological Reviews, 1983
- Structure of wild-type and mutant mouse β2-microglobulin genesCell, 1982
- Two kappa immunoglobulin genes are expressed in the myeloma S107Cell, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979