Forward scattering of light, X-rays and neutrons
- 1 May 1981
- journal article
- research article
- Published by Cambridge University Press (CUP) in Quarterly Reviews of Biophysics
- Vol. 14 (2), 141-172
- https://doi.org/10.1017/s0033583500002237
Abstract
The central points of this paper can now be summarized. We consider here, for simplicity only, vanishing particle concentration. In equilibrium sedimentation equation (6) applies. The density increment is a measurable quantity. It can either be introduced into equation (6) to calculateM2, or it can be analysed by equations (7) and (8) to provide additional information on specific volumes and solute interactions.Light scattering is determined by the analogous equation (20). The refractive index increment is also experimentally accessible and its structure (not considered here) is similar to that of the density increment. Small angle X-ray scattering is determined by equation (31) and the electron density increment which appears in this equation cannot be directly determined by experiment. Yet it can be obtained in straightforward fashion from the mass density increment, by equation (34). Similarly, in the case of neutron scattering (equation (38)), the scattering length density increment is obtained from the mass density increment by equation (40), or it may now be directly evaluated by neutron interferometry.Keywords
This publication has 37 references indexed in Scilit:
- Binding of additional histones to chromatin core particlesNature, 1978
- Bovine serum albumin in aqueous guanidine hydrochloride solutions. Preferential and absolute interactions and comparison with other systemsBiochemistry, 1977
- Comparison of neutron and X-ray scattering of dilute myoglobin solutionsJournal of Molecular Biology, 1975
- Étude du contraste et de son influence sur les déterminations de l'intensité diffusée et du rayon de giration dans les systèmes de macromolécules hétérogènesJournal de Physique, 1975
- Interpretation of equilibrium sedimentation measurements of proteins in guanidine hydrochloride solutions. Partial volumes, density increments, and the molecular weight of the subunits of rabbit muscle aldolaseBiochemistry, 1969
- An interpretation of the low-angle X-ray scattering of DNA solutionsJournal of Molecular Biology, 1968
- Light Scattering of Water, Deuterium Oxide, and Other Pure LiquidsThe Journal of Chemical Physics, 1965
- Multicomponent Polyelectrolyte Solutions. Part I. Thermodynamic Equations for Light Scattering and SedimentationThe Journal of Chemical Physics, 1962
- LIGHT-SCATTERING STUDIES OF COPOLYMERS: I. EFFECT OF HETEROGENEITY OF CHAIN COMPOSITION ON THE MOLECULAR WEIGHTCanadian Journal of Chemistry, 1958
- The Determination of Polymeric Molecular Weights by Light Scattering in Solvent-Precipitant SystemsThe Journal of Chemical Physics, 1946