Binding of the Human Glucocorticoid Receptor to Defined Regions in the Human Growth Hormone and Placental Lactogen Genes
- 1 December 1985
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 4 (6), 409-417
- https://doi.org/10.1089/dna.1985.4.409
Abstract
An in vitro competition assay was used to investigate whether binding sites for the human glucocorticoid receptor occur in the human genes for growth hormone (hGH) and placental lactogen (chorionic somatomammotropin, hCS). These genes display 95% sequence homology. Two receptor-binding regions were found in the hGH gene, one of which is located within 290 bp upstream, and one within 251 bp downstream from the transcription initiation site. Two binding regions homologous to those in the hGH gene were found in the hCS gene. The receptor-binding DNA fragment from the structural part of the genes, but not that from their promoter area, contained a sequence homologous to a 15-bp consensus sequence proposed earlier for the glucocorticoid receptor binding site. It is unlikely that the putative difference in glucocorticoid sensitivity between the hGH and hCS genes is accounted for by major differences in glucocorticoid receptor binding pattern.This publication has 28 references indexed in Scilit:
- Regulation of growth hormone messenger RNA synthesis by dexamethasone and triiodothyronineJournal of Molecular Biology, 1985
- Infectious and Selectable Retrovirus Containing an Inducible Rat Growth Hormone MinigeneScience, 1984
- Sequence-specific binding of glucocorticoid receptor to MTV DNA at sites within and upstream of the transcribed regionCell, 1983
- The glucocorticoid receptor binds to defined nucleotide sequences near the promoter of mouse mammary tumour virusNature, 1983
- Specific binding of the glucocorticoid-receptor complex to the mouse mammary tumor proviral promoter regionCell, 1982
- Regulated expression of human growth hormone genes in mouse cellsCell, 1982
- Long terminal repeats of endogenous mouse mammary tumour virus contain a long open reading frame which extends into adjacent sequencesNature, 1982
- Glucocorticoid receptor-mediated stimulation of 5′-nucleotidase in human lymphoblastoid IM-9 cellsFEBS Letters, 1980
- Human Growth Hormone: Complementary DNA Cloning and Expression in BacteriaScience, 1979
- The isolation and characterization of linked δ- and β-globin genes from a cloned library of human DNACell, 1978