Stimulation or Inhibition of Virus of Infected and Insect-Gall Tissues and Single-Cell Clones

Abstract

Tissue cultures from crown galls, insect galls and homologous normal tissue on many different plant species can usually be grown on relatively simple, chemically defined media though certain isolates require more complex balances of chemicals. In all cases concentrations of common metabolites are critical in stimulating or inhibiting growth. Clones of tissue derived from individual single cells from the tissue masses of crown-gall origin showed differences in growth rates, color, cell morphology, nutritional requirements and texture. Single-cell clones isolated from tobacco tissue either free from or infected with tobacco mosaic virus also showed variations in growth of single-cell clones, in susceptibility to virus infection, and conditions stimulating or inhibiting virus multiplication.