Isolation and Identification of Aldosterone from Adrenal Venous Blood

Abstract

A highly potent sodium-retaining steroid was isolated from adrenal venous blood and demonstrated to be identical with aldosterone with regard to: (1) infra-red spectrum, (2) chromatographic behavior of the parent compound and 2 derivatives, (3) biological activity in inducting sodium retention in adrenalectomized rats, (4) UV absorption and (5) sulfuric acid chromogen.