Comparison of Methods for Recovering Poliomyelitis Viruses from Human Sources.

Abstract

From human sources 104 fecal and oropharyngeal specimens were obtained directly and studied in 3 types of tissue cultures. The virus recovery rates among fecal samples were of the same order regardless of the type of tissue culture used. Under the conditions of the study, monkey kidney epithelial cell cultures were superior to HeLa or testicular cultures in primary isolation of poliomyelitis virus from oropharyngeal specimens. A comparison of tests with fecal samples in monkeys and in tissue culture indicated that the latter was as good as, if not better than, monkeys in detecting poliomyelitis virus.