Eukaryotic elongation factor 2 loses its non‐specific affinity for RNA and leaves polyribosomes as a result of ADP‐ribosylation
- 29 October 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 176 (2), 406-410
- https://doi.org/10.1016/0014-5793(84)81207-7
Abstract
ADP-ribosylation of rabbit reticulocyte elongation factor 2 (EF-2) catalyzed by the A fragment of diphtheria toxin leads to a loss of its non-specific affinity for RNA. The removal of the ADP-ribose residue from EF-2 in the reverse reaction with nicotinamide restores its affinity for RNA. ADP-ribosylation of EF-2 is accompanied by its dissociation from the complexes with mono- and polyribosomes detected in the rabbit reticulocyte lysate at low ionic strength. The loss of the non-specific affinity of EF-2 for RNA as a result of ADP-ribosylation and, as a consequence, its decompartmentation from polyribosomes is assumed to be a reason for the diphtheria toxin-induced inactivation of the factor in eukaryotic cells.Keywords
This publication has 28 references indexed in Scilit:
- Aminoacyl‐tRNA synthetases of rabbit reticulocytes with and without the ability to bind high‐Mr RNAFEBS Letters, 1982
- Protein kinase activity in RNA‐binding proteins of Amphibia oocytesFEBS Letters, 1982
- Difference in RNA‐binding ability between eukaryotic and prokaryotic elongation factors of translationFEBS Letters, 1978
- RNA-Binding Proteins of Rabbit Reticulocytes. Isolation and Electrophoretic CharacteristicsEuropean Journal of Biochemistry, 1978
- Translation factors of the wheat embryo extract are RNA‐binding proteinsFEBS Letters, 1978
- Eukaryotic messenger RNA and informosomesFEBS Letters, 1978
- RNA‐Binding proteins of rabbit reticulocytes contain the two elongation factors and some of the initiation factors of translationFEBS Letters, 1978
- Initiation of mammalian protein synthesisJournal of Molecular Biology, 1977
- Formation of a complex involving ADP‐ribosylated human translocation factor, guanosine nucleotide and ribosomesFEBS Letters, 1972
- Selective inhibition of the reactions catalyzed by ribosome‐specific transfer factors GFEBS Letters, 1971