Expression of glucagon‐like peptide 1 receptor in a murine C cell line Regulation of calcitonin gene by glucagon‐like peptide 1
- 16 September 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 393 (2-3), 248-252
- https://doi.org/10.1016/0014-5793(96)00895-2
Abstract
We have characterized, by RT-PCR amplification using specific primers, the presence of glucagon-like peptide-1 (GLP-I) receptor mRNA in CA-77 cells, a C cell line derived from a rat medullary thyroid carcinoma. Down-regulation of the GLP-1 receptor mRNA was observed after exposure of CA-77 C cells with GLP-1 (7-37). Increased secretion of both calcitonin gene-related peptide (CGRP) and calcitonin (CT) occurred after treatment with GLP-1 (7-37) associated with elevated steady-state levels of CGRP and CT mRNA. GLP-1 (7-37) increased cAMP formation in CA-77 cells in a dose-dependent manner; exendin (9-39), a GLP-1 receptor antagonist, inhibited cAMP production. The GLP-1 peptide which is produced by intestinal cells could be involved in the control of CT secretion through an entero-thyroidal axis implying GLP-1 receptor and increased CT gene expression.Keywords
This publication has 21 references indexed in Scilit:
- Regulation of glucagon and glucagon-like peptide-1 receptor messenger ribonucleic acid expression in cultured rat pancreatic islets by glucose, cyclic adenosine 3',5'-monophosphate, and glucocorticoidsEndocrinology, 1995
- Effects of 17β-estradiol on calcitonin and calcitonin-gene-related peptide secretions and contents in a murine medullary thyroid carcinoma C-cell line (CA-77)Reproduction Nutrition Development, 1995
- Cloning and functional expression of the human glucagon-like peptide-1 (GLP-1) receptorEndocrinology, 1993
- Stimulation of calcitonin/CGRP-I and CGRP-II gene expression by dibutyryl cAMP in a human medullary thyroid carcinoma (TT) cell lineMolecular and Cellular Endocrinology, 1993
- Potassium administration and calcitonin mRNA level in the ratMolecular and Cellular Endocrinology, 1988
- Studies of short-term secretion of peptides produced by alternative RNA processingMolecular and Cellular Endocrinology, 1987
- Insulinotropin: glucagon-like peptide I (7-37) co-encoded in the glucagon gene is a potent stimulator of insulin release in the perfused rat pancreas.Journal of Clinical Investigation, 1987
- Molecular forms of glucagon‐like peptides in manFEBS Letters, 1985
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- Effects of glucagon, dibutyryl cyclic 3′,5′-adenosine monophosphate, and theophylline on calcitonin secretion in vitroJournal of Clinical Investigation, 1970