A CHANGE IN OPTICAL ROTATION OF CREATINE-ATP TRANSPHOSPHORYLASE DURING ENZYME SUBSTRATE INTERACTION SUGGESTING AN ALTERATION IN CONFORMATION

Abstract
The specific rotation and rotatory dispersion of creatine-ATP-transphosphorylase has been measured and found to follow the single term Drude equation between 313 and 589m [mu]. The enzyme has been found to undergo a small but consistent increase in specific rotation only when in equilibrium with all of its substrates, which suggests a conformation change during activity.