Expression of Three Gastrula Cell Surface Glycoproteins during Embryonic and Larval Development in the Amphibian Pleurodeles waltlii. (amphibian/antibodies/cell surface glycoproteins/development)

Abstract

We have previously reported the identification of cell surface glycoproteins in Pleurodeles waltlii gastrulae. In an attempt to study the expression of three of these cell surface glycoproteins (proteins referred to 1, 11 and 14), we have produced monoclonal and polyclonal antibodies by immunizing mice with the spots of the three selected glycoproteins excised from 2D-gels. Expression of the three glycoproteins was detected on the surfaces of all cells during embryonic development. Before hatching, proteins, 1, 11 and 14 become expressed in a limited number of tissues.