MOUSE BRAIN PROTEIN COMPOSITION DURING POSTNATAL DEVELOPMENT: AN ELECTROPHORETIC ANALYSIS
- 1 November 1976
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 27 (5), 1163-1172
- https://doi.org/10.1111/j.1471-4159.1976.tb00323.x
Abstract
Changes in the concentrations of mouse brain proteins during postnatal maturation were characterized by a combination of subcellular fractionation and electrophoresis. Sodium dodecyl sulfate gel electrophoresis revealed changing protein concentrations in fractions enriched in nuclei, mitochondria plus synaptic endings, microsomes and cytosol. Postnatal maturational changes in protein concentrations were most pronounced in fractions of purified myelin membranes. The use of exponential gradient gels resulted in increased resolution of low MW myelin proteins. Nuclei treated with Triton X-100 exhibited no change in relative histone concentrations during brain maturation. Nonnuclear contamination of untreated nuclear fractions was shown to be a potential source of erroneous interpretations. Genetic products and sodium dodecyl sulfate polyacrylamide gel electrophoresis resolution were discussed.This publication has 47 references indexed in Scilit:
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