CELLULAR RETINOL-ACID AND RETINOIC ACID-BINDING PROTEINS IN TRANSFORMED MAMMALIAN-CELLS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 17 (10), 988-992
Abstract
Extracts prepared from several lines of transformed cells were examined for cellular binding proteins specific for retinoids. Extracts of human retinoblastoma cell line WERI-Rb1 contained a cellular binding protein specific for retinoic acid whereas extracts of human retinoblastoma cell line Y-79 contained cellular binding proteins for both retinol and retinoic acid. On purification the latter 2 binding proteins had properties similar to those of the corresponding proteins obtained from bovine retina. Smaller amounts of these binding proteins were detected in extracts of undifferentiated and differentiated neuroblastoma and McCoy cells. HeLa and rat glioma cells had no detectable amount of binding proteins. The 11-cis-retinal-binding protein present in extracts of human, rat and bovine retina was not found in any of the cell lines examined.