LIMITATIONS IN THE USE OF [3H]THYMIDINE INCORPORATION INTO DNA AS AN INDICATOR OF EPIDERMAL KERATINOCYTE PROLIFERATION IN VITRO
- 1 November 1979
- journal article
- research article
- Published by Wiley in Cell Proliferation
- Vol. 12 (6), 605-614
- https://doi.org/10.1111/j.1365-2184.1979.tb00180.x
Abstract
The validity of using the incorporation of [3H]thymidine into DNA as an indicator of epidermal keratinocyte proliferation in vitro was investigated. Other parameters of cell proliferation, direct count of cell number and measurement of DNA content consistently fail to correlate with changes in [3H]thymidine incorporation into DNA in primary and 1st passage cultures of rabbit and human epidermal keratinocytes. Maximum incorporation of [3H]thymidine precedes the active growth period by 3 days. Incorporation declines markedly during the proliferative period. Thymidine kinase activity decreases during the proliferative growth phase. Incorporation of another pyrimidine nucleotide precursor, [14C]aspartic acid, suggests that in epidermal keratinocytes in vitro the extent of utilization of the salvage and the de novo pathways may be inversely related. In such cases [3H]thymidine incorporation into TCA [trichloroacetic acid] precipitable material fails to reflect accurately cell proliferation.This publication has 13 references indexed in Scilit:
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