Monoclonal Antibodies Directed against Human Immunodeficiency Virus (HIV) gag Proteins with Specificity for Conserved Epitopes in HIV-1, HIV-2 and Simian Immunodeficiency Virus
- 1 August 1988
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 69 (8), 2109-2114
- https://doi.org/10.1099/0022-1317-69-8-2109
Abstract
Summary Monoclonal antibodies (MAbs) were raised against gag proteins of human immunodeficiency virus type 1 (HIV-1), strain HTLV-IIIB. One of 29 antibodies was specific for p17 of HIV-1. Twenty of 28 MAbs reactive with the major core protein p24 of HIV-1 showed cross-reactivity with HIV-2, and five of these also detected the corresponding antigens of simian immunodeficiency virus (SIVmac). The MAbs were reactive in several tests, i.e. ELISA, immunostaining of Western blots, immunofluorescence, alkaline phosphatase-anti-alkaline phosphatase immunocytochemistry and immunoelectron microscopy. The submembrane protein p17 was clearly localized within the virion.Keywords
This publication has 10 references indexed in Scilit:
- Monoclonal antibodies directed against a 130K glycoprotein of bovine herpesvirus 2 cross-react with glycoprotein B of herpes simplex virusVirus Research, 1988
- Fine structure of human immunodeficiency virus (HIV), immunolocalization of structural proteins and virus-cell relationMicron and Microscopica Acta, 1988
- Characterization of Monoclonal Antibodies Against the Human Immunodeficiency Virus (HIV) gag Products and Their Use in Monitoring HIV Isolate VariationJournal of General Virology, 1987
- Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteinsVirology, 1987
- Isolation of a New Human Retrovirus from West African Patients with AIDSScience, 1986
- Isolation of T-Cell Tropic HTLV-III-Like Retrovirus from MacaquesScience, 1985
- Complete nucleotide sequence of the AIDS virus, HTLV-IIINature, 1985
- Nucleotide sequence of the AIDS virus, LAVCell, 1985
- Detection, Isolation, and Continuous Production of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and Pre-AIDSScience, 1984
- Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes).Journal of Histochemistry & Cytochemistry, 1984