GTP-binding protein associated with amino acid binding proteins from olfactory epithelium of skate,Dasyatis pastinaca
- 18 July 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 234 (2), 471-474
- https://doi.org/10.1016/0014-5793(88)80140-6
Abstract
Amino acid binding protein (98 kDa) from olfactory epithelium of skate, Dasyatis pastinaca, form a stable complex with the 56 kDa protein. This complex dissociates in the presence of 10 μM GTPγS and 2 mM MgCl2. The 56 kDa protein has a steady-state GTPase activity (15 nmol/min per mg). Binding of amino acids to the 98 kDa protein specifically stimulates GTPase activity of the 56 kDa protein; half-maximal stimulation of GTPase activity is observed at 0.1 μM amino acid.Keywords
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