Evaluation of Molecular-Beacon, TaqMan, and Fluorescence Resonance Energy Transfer Probes for Detection of Antibiotic Resistance-Conferring Single Nucleotide Polymorphisms in Mixed Mycobacterium tuberculosis DNA Extracts
Open Access
- 1 October 2006
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 44 (10), 3826-3829
- https://doi.org/10.1128/jcm.00225-06
Abstract
The ability of fluorescence resonance energy transfer, molecular-beacon, and TaqMan probes to detect single nucleotide polymorphism (SNP) in the presence of a wild-type allele was evaluated using drug resistance-conferring SNPs in mixed Mycobacterium tuberculosis DNA. It was found that both the absolute quantity and the ratio of alleles determine the detection sensitivity of the probe systems.Keywords
This publication has 18 references indexed in Scilit:
- The use of real-time PCR methods in DNA sequence variation analysisClinica Chimica Acta; International Journal of Clinical Chemistry, 2005
- Drug Resistance Evolution of a Mycobacterium tuberculosis Strain from a Noncompliant PatientJournal of Clinical Microbiology, 2005
- Antibiotic resistance may affect alkali decontamination of specimens containing mycobacteriaDiagnostic Microbiology and Infectious Disease, 2004
- Molecular Characterization of Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates in LithuaniaAntimicrobial Agents and Chemotherapy, 2003
- Strategies against multidrug-resistant tuberculosisEuropean Respiratory Journal, 2002
- A review on SNP and other types of molecular markers and their use in animal geneticsGenetics Selection Evolution, 2002
- High Prevalence of KatG Ser315Thr Substitution among Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates from Northwestern Russia, 1996 to 2001Antimicrobial Agents and Chemotherapy, 2002
- Detection of Rifampin Resistance in Mycobacterium tuberculosis in a Single Tube with Molecular BeaconsJournal of Clinical Microbiology, 2001
- Double infection with a Resistant and a Multidrug-Resistant Strain of Mycobacterium tuberculosisEmerging Infectious Diseases, 2000
- Multidrug-Resistant Mycobacterium tuberculosis: Molecular PerspectivesEmerging Infectious Diseases, 1998