Effect of Triiodothyronine on Rat Liver Chromatin Protein Kinase
- 1 February 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 62 (1), 109-115
- https://doi.org/10.1111/j.1432-1033.1976.tb10103.x
Abstract
Injection of triiodothyronine to rats stimulates protein kinase activity in liver chromatin nonhistone proteins. A significant increase was found after 2 daily injections. A 4-fold increase was observed with the purified enzyme after 8 daily injections of the hormone. No variations were observed in cytosol protein kinase activity. Electrophoretic pattern, effect of heat denaturation, effect of p-hydroxymercuribenzoate seem to indicate that the enzyme present in treated rats is not identical to the enzyme in control animals, which suggests that thyroid hormone has induced nuclear protein kinase. Diiodothyronine, 3,3'',5''-triiodothyronine have no effect on protein kinase. Chromatin non-histone proteins isolated from rats injected with triiodothyronine incorporated more 32P when incubated with [.gamma.-32P]ATP than the chromatin proteins from untreated rats. Thyroidectomy reduced the in vitro 32P incorporation. Some of the biological activity of thyroid hormone could be mediated through its effect on chromatin non-histone proteins.This publication has 29 references indexed in Scilit:
- The effect of triiodothyronine on myocardial protein kinasesMolecular and Cellular Biochemistry, 1975
- Rat myocardial protein kinases: I. CytosolBiochimie, 1975
- Chromosomal non histone proteins and nuclear protein kinasesBiochimie, 1974
- In vitro triiodothyronine binding to non-histone proteins from rat liver nucleiBiochemical and Biophysical Research Communications, 1974
- Multiple forms of protein kinase in liver cell. I — CytosolBiochimie, 1973
- Effects of thyroid hormones on phosphorylation of liver ribosomal proteins and on protein phosphokinase activityFEBS Letters, 1972
- Changes in Hepatic Nuclear DNA-dependent RNA Polymerase caused by Growth Hormone and TriiodothyronineNature, 1971
- Rat liver non-histone proteins: Correlation between protein kinase activity and activation of RNA synthesisBiochemical and Biophysical Research Communications, 1971
- Phosphoprotein kinases associated with rat liver chromatinBiochemical and Biophysical Research Communications, 1971
- Isolation of pure and unaltered liver nuclei morphology and biochemical compositionExperimental Cell Research, 1956